Circular RNA (circRNA) is a noncoding RNA class with important implications for gene expression regulation, mostly by interaction with…
Protocols
Lab Protocols
PLOS ONE is committed to pushing the boundaries of Open Science by improving the reproducibility and transparency of scientific research. Earlier this year we launched Lab Protocols in collaboration with protocols.io. Lab Protocols offer authors the opportunity to share their peer reviewed step-by-step protocols with the community whilst receiving credit for their contributions. In this collection, we are highlighting the first set of Lab Protocols that PLOS ONE has published from across our broad scope, from molecular biology, biotechnology, structural biology to archaeology.
PLOS ONE
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PLOS ONE Generation of full-length circular RNA libraries for Oxford Nanopore long-read sequencing
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PLOS ONE Modular automated bottom-up proteomic sample preparation for high-throughput applications
Manual proteomic sample preparation methods limit sample throughput and often lead to poor data quality when thousands of samples must be…
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Image credit10.1371/journal.pone.0263071 by John Doe, CC BY 4.0PLOS ONE Comparison of analytical methods for the fatty acid profile in ewes’ milk
Studies comparing methodologies for fatty acids are very important, since they can influence the quality and final quantification of the…
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PLOS ONE Crosslinking assay to study a specific cargo-coat interaction through a transmembrane receptor in the secretory pathway
Intracellular trafficking through the secretory organelles depends on transient interactions between cargo proteins and transport…
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PLOS ONE Biolayer interferometry for DNA-protein interactions
Biolayer interferometry (BLI) is a widely utilized technique for determining macromolecular interaction dynamics in real time. Using…
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PLOS ONE Engineering solutions for biological studies of flow-exposed endothelial cells on orbital shakers
Shear stress is extremely important for endothelial cell (EC) function. The popularity of 6-well plates on orbital shakers to impose shear…
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PLOS ONE Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencing
The SARS-CoV-2 responsible for the ongoing COVID pandemic reveals particular evolutionary dynamics and an extensive polymorphism, mainly…
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PLOS ONE Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice
Interaction of Clostridioides difficile spores with the intestinal mucosa contributes to the persistence and recurrence of the infection.…
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PLOS ONE Development of a real-time PCR assay to detect the single nucleotide polymorphism causing Warmblood Fragile Foal Syndrome
Warmblood Fragile Foal syndrome (WFFS) is an autosomal recessive condition that affects the maturation of collagen in affected foals.…
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PLOS ONE Colorimetric determination of urea using diacetyl monoxime with strong acids
Urea is a byproduct of the urea cycle in metabolism and is excreted through urine and sweat. Ammonia, which is toxic at low levels, is…
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PLOS ONE A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm
The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent…
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PLOS ONE Assay for dual cargo sorting into endoplasmic reticulum exit sites imaged by 3D Super-resolution Confocal Live Imaging Microscopy (SCLIM)
Understanding how in eukaryotic cells thousands of proteins are sorted from each other through the secretory pathway and delivered to…
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PLOS ONE Protocol for safe, affordable, and reproducible isolation and quantitation of SARS-CoV-2 RNA from wastewater
The following protocol describes our workflow for processing wastewater with the goal of detecting the genetic signal of SARS-CoV-2. The…
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PLOS ONE Structural analysis of the GPI glycan
Glycosylphosphatidylinositol (GPI) anchoring of proteins is an essential post-translational modification in all eukaryotes that occurs at…
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PLOS ONE Determination of the lipid composition of the GPI anchor
In eukaryotic cells, a subset of cell surface proteins is attached by the glycolipid glycosylphosphatidylinositol (GPI) to the external…
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PLOS ONE High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing
Rapid advancements in long-read sequencing technologies have transformed read lengths from bps to Mbps, which has enabled chromosome-scale…
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PLOS ONE Vectorial application for the illustration of archaeological lithic artefacts using the “Stone Tools Illustrations with Vector Art” (STIVA) Method
Lithic illustrations are often used in scientific publications to efficiently communicate the technological and morphological…