Biology & Life Sciences

Advances in HIV Mucosal Immunology: Challenges and Opportunities

Published August 15, 2018 Partnered Collections, Special Issues

Without reliable methods to evaluate how the mucosal immune system responds to an experimental HIV vaccine, important information about how well that vaccine worked is missed. The HIV Mucosal Immunology Group (MIG) was established to address the challenge of assessing the impact of potential HIV vaccines on the mucosal immune system. The MIG comprises of expert scientists who are coordinating their efforts to improve mucosal sampling, specimen storage and assay technologies. This collection reports the results of those efforts, providing important, practical details on studying immune responses in the genital and rectal mucosa.

Image Credit: Artistic rendition of immune cells and potential HIV targets in the rectal mucosal. Yang, Ochoa, Preza & Anton, 2014

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From the Blogs

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Yang, Ochoa, Preza & Anton, 2014

Speaking of Medicine Researchers Follow the Path of HIV and Prevention Interventions
October 31, 2014 Charlene Dezzutti
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Yang, Ochoa, Preza & Anton, 2014

Speaking of Medicine Advances in HIV Mucosal Immunology: Challenges and Opportunities
April 29, 2014 Florian Hladik

Research

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10.1371/journal.pone.0200653

PLOS ONE Cryopreservation of human mucosal tissues
July 30, 2018 Sean M. Hughes, April L. Ferre, Sarah E. Yandura, Cory Shetler, Chris A. R. Baker, Fernanda Calienes, Claire N. Levy, Rena D. Astronomo, Zhiquan Shu, Gretchen M. Lentz, Michael Fialkow, Anna C. Kirby, M. Juliana McElrath, Elizabeth Sinclair, Lisa C. Rohan, Peter L. Anderson, Barbara L. Shacklett, Charlene S. Dezzutti, Dayong Gao, Florian Hladik
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10.1371/journal.pone.0160487

PLOS ONE Transient Peripheral Immune Activation follows Elective Sigmoidoscopy or Circumcision in a Cohort Study of MSM at Risk of HIV Infection
August 18, 2016 Javier R. Lama, Shelly T. Karuna, Shannon P. Grant, Edith M. Swann, Carmela Ganoza, Patricia Segura, Silvia M. Montano, Martin Lacherre, Stephen C. De Rosa, Susan Buchbinder, Jorge Sanchez, M. Juliana McElrath, Maria P. Lemos, HVTN 914 Study Team
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10.1371/journal.pone.0156293

PLOS ONE Cryopreservation of Human Mucosal Leukocytes
May 27, 2016 Sean M. Hughes, Zhiquan Shu, Claire N. Levy, April L. Ferre, Heather Hartig, Cifeng Fang, Gretchen Lentz, Michael Fialkow, Anna C. Kirby, Kristina M. Adams Waldorf, Ronald S. Veazey, Anja Germann, Hagen von Briesen, M. Juliana McElrath, Charlene S. Dezzutti, Elizabeth Sinclair, Chris A. R. Baker, Barbara L. Shacklett, Dayong Gao, Florian Hladik
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10.1371/journal.pone.0131906

PLOS ONE Randomized Cross-Sectional Study to Compare HIV-1 Specific Antibody and Cytokine Concentrations in Female Genital Secretions Obtained by Menstrual Cup and Cervicovaginal Lavage
July 6, 2015 Derseree Archary, Lenine J. Liebenberg, Lise Werner, Sahil Tulsi, Nelisile Majola, Nivashnee Naicker, Sarah Dlamini, Thomas J. Hope, Natasha Samsunder, Salim S. Abdool Karim, Lynn Morris, Jo-Ann S. Passmore, Nigel J. Garrett
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10.1371/journal.pone.0127091.t001

PLOS ONE Impact of Bacterial Vaginosis, as Assessed by Nugent Criteria and Hormonal Status on Glycosidases and Lectin Binding in Cervicovaginal Lavage Samples
May 26, 2015 Bernard J. Moncla, Catherine A. Chappell, Lara K. Mahal, Brian M Debo, Leslie A Meyn, Sharon L. Hillier
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10.1371/journal.pone.0126454

PLOS ONE Exploring the Feasibility of Multi-Site Flow Cytometric Processing of Gut Associated Lymphoid Tissue with Centralized Data Analysis for Multi-Site Clinical Trials
May 26, 2015 Ian McGowan, Peter A. Anton, Julie Elliott, Ross D. Cranston, Kathryn Duffill, Andrew D. Althouse, Kevin L. Hawkins, Stephen C. De Rosa
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10.1371/journal.pone.0127021

PLOS ONE Studying the Effects of Reproductive Hormones and Bacterial Vaginosis on the Glycome of Lavage Samples from the Cervicovaginal Cavity
May 20, 2015 Linlin Wang, Sujeethraj Koppolu, Catherine Chappell, Bernard J. Moncla, Sharon L. Hillier, Lara K. Mahal
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10.1371/journal.pone.0122723

PLOS ONE T Lymphocyte Density and Distribution in Human Colorectal Mucosa, and Inefficiency of Current Cell Isolation Protocols
April 9, 2015 Gloria Cuevas Preza, Otto O. Yang, Julie Elliott, Peter A. Anton, Maria T. Ochoa
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10.1371/journal.pone.0110228

PLOS ONE Acceptability and Feasibility of Repeated Mucosal Specimen Collection in Clinical Trial Participants in Kenya
October 31, 2014 Gloria Omosa-Manyonyi, Harriet Park, Gaudensia Mutua, Bashir Farah, Philip Bergin, Dagna Laufer, Jennifer Lehrman, Kundai Chinyenze, Burc Barin, Pat Fast, Jill Gilmour, Omu Anzala
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10.1371/journal.pone.0106196

PLOS ONE A Multi-Compartment Single and Multiple Dose Pharmacokinetic Comparison of Rectally Applied Tenofovir 1% Gel and Oral Tenofovir Disoproxil Fumarate
October 28, 2014 KUO-HSIUNG Yang, Craig Hendrix, Namandje Bumpus, Julie Elliott, Karen Tanner, Christine Mauck, Ross Cranston, Ian McGowan, Nicola Richardson-Harman, Peter Anton, Angela Kashuba
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10.1371/journal.pone.0111507

PLOS ONE Correlation between Compartmental Tenofovir Concentrations and an Ex Vivo Rectal Biopsy Model of Tissue Infectibility in the RMP-02/MTN-006 Phase 1 Study
October 28, 2014 Nicola Richardson-Harman, Craig Hendrix, Namandjé Bumpus, Christine Mauck, Ross Cranston, Kuo Yang, Julie Elliott, Karen Tanner, Ian McGowan, Angela Kashuba, Peter Anton
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10.1371/journal.pone.0108954

PLOS ONE The Inner Foreskin of Healthy Males at Risk of HIV Infection Harbors Epithelial CD4+ CCR5+ Cells and Has Features of an Inflamed Epidermal Barrier
September 30, 2014 Maria Lemos, Javier Lama, Shelly Karuna, Youyi Fong, Silvia Montano, Carmela Ganoza, Raphael Gottardo, Jorge Sanchez, M McElrath
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10.1371/journal.pone.0101863

PLOS ONE HIV-1 Specific IgA Detected in Vaginal Secretions of HIV Uninfected Women Participating in a Microbicide Trial in Southern Africa Are Primarily Directed Toward gp120 and gp140 Specificities
July 23, 2014 Kelly Seaton, Lamar Ballweber, Audrey Lan, Michele Donathan, Sean Hughes, Lucia Vojtech, M. Anthony Moody, Hua-Xin Liao, Barton Haynes, Christine Galloway, Barbra Richardson, Salim Abdool Karim, Charlene Dezzutti, M. Juliana McElrath, Georgia Tomaras, Florian Hladik
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10.1371/journal.pone.0102585

PLOS ONE Development of HIV-1 Rectal-Specific Microbicides and Colonic Tissue Evaluation
July 15, 2014 Charlene Dezzutti, Julie Russo, Lin Wang, Kaleab Abebe, Jie Li, David Friend, Ian McGowan, Lisa Rohan
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10.1371/journal.pone.0100820

PLOS ONE A Comparative Proteomic Analysis of the Soluble Immune Factor Environment of Rectal and Oral Mucosa
June 30, 2014 Laura Romas, Klara Hasselrot, Lindsay Aboud, Kenzie Birse, T. Ball, Kristina Broliden, Adam Burgener
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10.1371/journal.pone.0088621

PLOS ONE Differential Blood and Mucosal Immune Responses against an HIV-1 Vaccine Administered via Inguinal or Deltoid Injection
February 18, 2014 Otto Yang, F Javier Ibarrondo, Charles Price, Lance Hultin, Julie Elliott, Patricia Hultin, Roger Shih, Mary Ann Hausner, Hwee Ng, Jennifer Hoffman, Beth Jamieson, Peter Anton
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10.1371/journal.pone.0085675

PLOS ONE Optimizing Viable Leukocyte Sampling from the Female Genital Tract for Clinical Trials: An International Multi-Site Study
January 15, 2014 Lyle McKinnon, Sean Hughes, Stephen De Rosa, Jeffrey Martinson, Jill Plants, Kirsten Brady, Pamela Gumbi, Devin Adams, Lucia Vojtech, Christine Galloway, Michael Fialkow, Gretchen Lentz, Dayong Gao, Zhiquan Shu, Billy Nyanga, Preston Izulla, Joshua Kimani, Steve Kimwaki, Alfred Bere, Zoe Moodie, Alan Landay, Jo-Ann Passmore, Rupert Kaul, Richard Novak, M. McElrath, Florian Hladik
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10.1371/journal.pone.0079505

PLOS ONE Unbiased Proteomics Analysis Demonstrates Significant Variability in Mucosal Immune Factor Expression Depending on the Site and Method of Collection
November 14, 2013 Kenzie Birse, Adam Burgener, Garrett Westmacott, Stuart McCorrister, Richard Novak, T Blake Ball
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10.1371/journal.pone.0075620

PLOS ONE HIV-1 Nef Sequence and Functional Compartmentalization in the Gut Is Not Due to Differential Cytotoxic T Lymphocyte Selective Pressure
September 13, 2013 Martha Lewis, Patricia Frohnen, F. Ibarrondo, Diane Reed, Varun Iyer, Hwee Ng, Julie Elliott, Otto Yang, Peter Anton

Web Resources

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MIG

MIG HIV Mucosal Immunology Group
October 20, 2015 Mucosal Immunology Group
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Global HIV Vaccine Enterprise

Global HIV Vaccine Enterprise Capturing Participant Information for Mucosal Sampling: An Investigator’s Guide
April 29, 2014 Global HIV Vaccine Enterprise

Collection Image

An artistic rendition of Lymphocytes (orange), macrophages (purple), dendritic cells (green) and Langerhans-like cells (red) overlaid onto a confocal microscopic image from a sectioned rectal mucosal biopsy, using DAPI-staining of cell nuclei.

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